Table 2. Working detection ranges of standard curves for 5 tumor markers by MFCA and ELISA.

(E: ELISA; Mm: MFCA multiplexed; Ms: MFCA single. 50% value was the halfway point between the lower and upper ranges).

	Ms	Lower Mm	E	Ms	50% Mm	E	Ms	Upper Mm	E	Ms	Total Mm	E
AFP	0.016	0.016	0.004	0.30	0.36	0.02	16	16	4	1024×	1024×	256×
CEA	0.004	0.004	0.004	0.16	0.20	0.02	64	64	4	4096×	4096×	256×
CA19–9	0.063	0.063	0.016	2.1	2.0	0.22	64	64	4	1024×	1024×	256×
CA24–2	0.016	0.016	0.001	0.46	0.40	0.009	64	64	0.25	1024×	1024×	256×
CA72–4	0.004	0.004	0.001	0.22	0.16	0.055	64	64	4	4096×	4096×	256×