Table 4.
Characteristics | Antibody-based |
---|---|
Principle | Antibody-antigen interaction |
Required reagents/information | antibody pairs |
Quantification | Flow cytometry and others |
Multiplexicity | 1–100 |
Clinical diagnostic test | Yes |
Sample enrichment | No |
Sensitivity | >sub ng/mL–pg/mL |
Reproducibility | Excellent |
Assay development | Time and resource demanding |
Consumable | High demand |
Robustness | Yes |
Throughput | High |
Matrix effect | Yes |
Sample manipulation | No |
Automation | Yes |