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. 2018 Sep 24;13(9):e0204209. doi: 10.1371/journal.pone.0204209

Fig 7. The proposed model of the role of L-Plastin (LPL) in nascent sealing zones (NSZs) formation.

Fig 7

TNF-α signaling regulates the phosphorylation of LPL on serine residues which mediate the actin bundling process involved in the formation of NSZs. Osteoclasts treated with an inhibitor to αv or siRNA to β3 reduced the formation of matured sealing rings but not NSZs [ref. 3]; broken arrows). Therefore, NSZ formation occurs independently of integrin αvβ3 signaling. We show in this paper that sNT-LPL (P1) peptide has the potential to block the serine phosphorylation of endogenous LPL competitively. Inhibition of phosphorylation attenuates the actin bundling process involved in NSZs formation. Failure of formation of NSZs has an impact on the formation of sealing rings formation and bone resorption (solid arrows). We suggest that these NSZs are the presumable precursor zones for fully functional sealing rings. LPL is a possible attractive target for pharmacologic regulation of osteoclast sealing ring formation via blocking the formation of NSZs by LPL.