Table 1. Antibodies used in the study.
| Antibody | Species | Immunising antigen | Source |
|---|---|---|---|
| Anti-NKCC2 | Rabbit polyclonal | Rat N-terminus aa 33–55 | Stress-Marq |
| Anti-pNKCC2-T101/105 | Rabbit polyclonal | Human N-terminus aa 99–110 | Fraser et al [26] |
| Anti-pNKCC2-S130 | Rabbit polyclonal | Mouse N-terminus aa 121–130 | Cook et al [27] |
| Anti-NCC | Rabbit polyclonal | Rat N-terminus aa 74–95 | Abcam |
| Anti-pNCC-T60 | Rabbit polyclonal | Human N-terminus aa 54–66 | Fraser et al [26] |
| Anti-α-ENaC | Rabbit polyclonal | Rat N-terminus aa 46–68 |
Stress Marq |
| Anti-γ-ENaC | Rabbit polyclonal | Rat C-terminus aa 629–650 | Stress Marq |
| Anti-CD9 | Rabbit monoclonal | Human around aa 178 | Cell Signaling Technology |