Table 3. Detection of bands for phosphorylated forms of NKCC2 and NCC, and for ENaC proteins.
| Species | PE | NP | NC | P-value* |
|---|---|---|---|---|
| NKCC2-pS130 | 23/24 | 6/24 | 6/20 | <0.001# |
| NKCC2-pT101/105 | 15/24 | 21/24 | 16/20 | n.s. |
| NCC -pT60 | 11/24 | 21/24 | 18/20 | <0.001# |
| α-ENaC (total) | 20/24 | 12/24 | 13/21 | <0.05# |
| α-ENaC 90–100 kD | 2/24 | 3/24 | 4/21 | n.s. |
| α-ENaC 75 kD | 18/24 | 2/24 | 2/21 | <0.001# |
| α-ENaC 50 kD | 14/24 | 7/24 | 11/21 | n.s. |
| γ-ENaC (total) | 18/24 | 15/24 | 12/21 | n.s. |
| γ-ENaC 90–100 kD | 0/24 | 5/24 | 3/21 | n.s. |
| γ-ENaC 75 kD | 9/24 | 8/24 | 8/21 | n.s. |
| γ-ENaC 57–62 kD | 11/24 | 1/24 | 0/21 | <0.001$ |
| γ-ENaC 50 kD | 8/24 | 1/24 | 4/21 | n.s. |
The numerator is the number with a detectable band, and the denominator is the total number of samples analysed. One NC subject with missing pNKCC2 and pNCC data. n.s. = not significant.
*P-value refers to a 2x3 way contingency table comparing all groups analysed by or
#Chi-square or
$Fisher’s exact test.