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. 2018 Sep 12;14(9):e1007570. doi: 10.1371/journal.pgen.1007570

Fig 7. Overexpression of CBF-1 decreases recruitment of WCC to the C-box.

Fig 7

(A, B) Rhythmic binding of A) WC-1 and B) WC-2 to the C-box is decreased by overexpression of Myc-CBF-1. (C) ChIP assays showing that the binding of CBF-1 to C-box increased in wild-type strain with overexpression of CBF-1. (D) Race tube assays showing that overexpression of the mutant CBF-1 has no effect on circadian conidiation phenotype. (E, F) ChIP assays showing that the binding of E) WC-1 and F) WC-2 decreased in the wild-type strain upon overexpression of the wild-type CBF-1 but not mutant CBF-1 at DD14. All error bars represent the ±S.D. (n = 3; *P<0.05, **P<0.01, and ***P<0.001; two-tailed t-test). (G, H) Immunoprecipitation assays using G) WC-1 antiserum and H) WC-2 antiserum showing that different versions of CBF-1 proteins interact with WC-1 and WC-2 in vivo.