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. Author manuscript; available in PMC: 2019 Dec 5.
Published in final edited form as: Mol Cell Endocrinol. 2018 Jun 2;477:57–69. doi: 10.1016/j.mce.2018.06.001

Fig. 5.

Fig. 5

LIP expression induces endogenous PRA and PRB expression. Transient transfections of the LIP expression vector were carried out in MC7-L1 cells. (A) Immunofluorescent detection of PRA (green) and C/EBPβ (red) in cells transfected for LIP expression (LIP) and in cells transfected with empty pcDNA3.1 vector (Ctrl). Nuclei were counterstained with DAPI (blue). 20× magnification; scale bar, 50 µm. (B) The immunofluorescence of PRA and PRB were measured in cells showing LIP overexpression after transfection with LIP expression vector (LIP) in comparison to the level of immunofluorescence in cells transfected with empty vector (Ctrl). Control transfections showed no change in PR expression compared to untransfected cells (data not shown). Values from LIP overexpressing cells were normalized to a relative value of 1.0 for cells transfected with empty vector. The data presented are the means of at least three experiments with SEM. Approximately 100 LIP overexpressing cells were scored in each experiment. p was calculated by Student’s t-Test in comparison to the control transfection. **, p<0.05. (C) The quantities of PR mRNA were measured by qRT-PCR in cells transfected for LIP expression (LIP) in comparison to cells transfected with empty vector (Ctrl). Values were normalized to a relative value of 1.0 for cells receiving the empty vector control. The data presented are the means of nine experiments with SEM. p was calculated by Student’s t-Test in comparison to the control transfection. **, p<0.05.