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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: J Invest Dermatol. 2018 Mar 30;138(10):2253–2263. doi: 10.1016/j.jid.2018.03.1521

Figure 6. Repression of Rasa1, Spred1, Spred2 and SPRY4 can rescue the effects of miR-31 knockdown in keratinocytes.

Figure 6.

(a) In vitro scratch assays assessing the migration rate of HaCaT cells transfected with miR-31 inhibitor, inhibitor control and/or siRNAs for Rasa1, Spred1, Spred2 or SPRY4 for 24 hours. Representative images are shown at indicated time points. (b) Statistical analysis of scratch assay wound closure from panel (a). (c) WST-8 assays on HaCaT cells transfected with miR-31 inhibitor, inhibitor control and/or siRNA for Rasa1, Spred1, Spred2 or SPRY4. n = 3 biological replicates for a-c. ** p < 0.01 and * p < 0.05.