Figure 1.

αBc and CαBc inhibit aggregation of αSyn and K19. A, domain architecture of αBc. The CαBc is flanked by a flexible NR and a flexible CT containing a conserved IPI motif. B, ThT kinetics of αSyn aggregation inhibited by αBc (top) and CαBc (bottom), respectively. C, negative-stain EM images of αSyn (top) and K19 (bottom) fibrils with and without αBc/CαBc at different concentrations. D, comparison of chaperone activity of αBc and CαBc for preventing aggregation of αSyn (left) and K19 (right). The ThT value was taken at the 55-h time point from the ThT kinetics curves. Error bars correspond to mean ± S.E. with n = 3. * indicates p < 0.05, and *** indicates p < 0.005. a.u., absorbance units.