Rem2 binds to CaMKII.
A, mass spectrometry data identifying CaMKII isozymes retained by a Rem2 affinity matrix after loading with total brain lysate from P14 WT rats. The number of unique and total peptides and the percentage of the protein sequence covered for each isozyme are shown. B, coimmunoprecipitation of Rem2 and CaMKIIα from HEK293T cells transfected with HA-tagged Rem2, myc-tagged CaMKIIα, or both. Rem2 was immunoprecipitated using an anti-HA antibody. Anti-myc and anti-HA immunoblotting was performed to detect CaMKIIα and Rem2, respectively. C, coimmunoprecipitation of Rem2 and CaMKIIα from mouse brain lysates using goat anti-Rem2 antibody. Immunoblotting was performed using rabbit anti-Rem2 and mouse anti-CaMKIIα antibodies to detect Rem2 and CaMKIIα, respectively. The numbers represent the position of protein molecular mass standards in kDa. IP, immunoprecipitation; IB, immunoblotting. The experiment was repeated three times.