Figure 7.

A peptide based on amino acids 68–88 from the N terminus of Rem2 is a low-affinity CaMKII inhibitor. A, scheme depicting a 21-amino-acid peptide corresponding to the mouse Rem2 sequence between amino acid residues 68 and 88. The CaMKII pseudosubstrate sequence is underlined. B, total ATP consumption by purified CaMKIIα using syntide-2 as substrate (200 μm) (black squares) and in the presence of a 0.5 or 150 μm concentration of the peptide described in A (green circles and red squares, respectively). Samples devoid of syntide-2 were used as negative controls (gray triangles). C, quantification of CaMKIIα kinase activity shown in B (n = 6; error bars indicate standard deviations; ****, p < 0.0001, one-way ANOVA with Tukey's test).