Table 1.
EPR simulation parameters of spectra from WT and KpDyP variants (experimental error: ±0.01 for the g values, ±0.0005 for the E/D ratio, and ±1% for the contribution) compared with EPR data of other heme peroxidases
HRP, horseradish peroxidase; MtKatG, catalase-peroxidase from M. tuberculosis; MaCld, chlorite dismutase from Magnetospirillum sp.; CCld, chlorite dismutase from the cyanobacterium Cyanothece sp. PCC7425; TcDyP, dye-decolorizing peroxidase from T. curvata; AauDyP, dye-decolorizing peroxidase from A. auricula-judae; NdCld, chlorite dismutase from N. defluvii; RjDyP, dye-decolorizing peroxidase from R. jostii RHA1; TBD, to be determined; NR, not reported.
| gxeff | gyeff | gzeff | gx | gy | gz | E/D | I | |
|---|---|---|---|---|---|---|---|---|
| % | ||||||||
| KpDyP WT (this work) | ||||||||
| HS1 | 6.44 | 5.12 | 1.98 | 1.93 | 1.93 | 2 | 0.0288 | 77.3 |
| HS2 | 6.25 | 5.41 | 1.99 | 1.95 | 1.95 | 2 | 0.0178 | 22 |
| Radical | TBD | TBD | TBD | 0.7 | ||||
| KpDyP R232A (this work) | ||||||||
| HS1 | 6.06 | 5.69 | 1.99 | 1.96 | 1.96 | 2 | 0.0076 | 67.2 |
| HS2 | 6.2 | 5.52 | 1.99 | 1.95 | 1.95 | 2 | 0.0148 | 23.9 |
| HS3 | 6.05 | 5.88 | 2 | 1.99 | 1.99 | 2 | 0.0036 | 8.9 |
| KpDyP D143A (this work) | ||||||||
| HS1 | 5.99 | 5.85 | 2 | 1.97 | 1.97 | 2 | 0.0029 | 40.7 |
| HS2 | 6.13 | 5.69 | 1.94 | 1.97 | 1.97 | 2 | 0.0097 | 48.2 |
| HS3 | 6.43 | 5.15 | 1.97 | 1.94 | 1.94 | 2 | 0.0279 | 11.1 |
| KpDyP D143A/R232A (this work) | ||||||||
| HS1 | 6.13 | 5.61 | 1.99 | 1.96 | 1.96 | 2 | 0.0086 | 91.7 |
| HS2 | 6.49 | 5.21 | 1.98 | 1.96 | 1.96 | 2 | 0.0220 | 8.3 |
| HRP (65) | ||||||||
| HS | 6.35 | 5.65 | 2 | NR | NR | NR | NR | 100 |
| MtKatG (29) | ||||||||
| HS1 | 5.86 | 5.86 | 2 | NR | NR | NR | NR | NR |
| HS2 | 6.03 | 5.55 | 2 | NR | NR | NR | NR | NR |
| HS3 | 6.35 | 5.37 | 2 | NR | NR | NR | NR | NR |
| HS4 | 6.49 | 5.1 | 2 | NR | NR | NR | NR | NR |
| MaCld (30) | ||||||||
| HS1 | 6.72 | 5.05 | 1.96 | 1.97 | 1.97 | 2 | 0.0380 | 6.5 |
| HS2 | 6.29 | 5.45 | 1.99 | 1.97 | 1.97 | 2 | 0.0177 | 93.5 |
| CCld (32) | ||||||||
| HS1 | 5.93 | 5.8 | 2 | NR | NR | NR | 0.002 | 62 |
| HS2 | 6.05 | 5.65 | 2 | NR | NR | NR | 0.008 | 28 |
| LS | 2.8 | 2.28 | 1.85 | 10 | ||||
| TcDyP A classa (23) | ||||||||
| HS | 6.2 | 5.5 | 2 | NR | NR | NR | NR | 100 |
| AauDyP A class pH 3a (22) | ||||||||
| HS1 | 5.9 | 5.9 | 2 | NR | NR | NR | NR | NR |
| HS2 | 6.2 | 5.4 | 2 | NR | NR | NR | NR | NR |
| NdCld (66) | ||||||||
| HS1 | 5.50 | 6.17 | 1.99 | NR | NR | NR | 0.014 | 49 |
| HS2 | 5.95 | 5.95 | 1.99 | NR | NR | NR | NR | 19 |
| LS1 | 2.98 | 2.25 | 1.88 | 15 | ||||
| LS2 | 3.15 | 2.25 | 1.80 | 16 | ||||
| RjDyP A classa (6) | ||||||||
| HS | 6.32 | 5.45 | 1.97 | NR | NR | NR | NR | NR |
| RjDyP B classa (6) | ||||||||
| HS | 6.09 | 5.45 | 1.99 | NR | NR | NR | NR | NR |
a Readout values.