Figure 4.

Atherosclerotic lesion analysis, weights, and blood cell lipids of Neu1^hypoApoe^−/− mice transplanted with Neu1^hypoApoe^−/− or Apoe^−/− BM. Aortic sinus sections of male Neu1^hypoApoe^−/− mice transplanted with male Apoe^−/− BM or male Neu1^hypoApoe^−/− BM were stained with Oil Red O and were quantified for the cross-sectional area (A) and volume (B) of atherosclerotic lesions. Transplantation of male Apoe^−/− BM into male Neu1^hypoApoe^−/− mice (n = 9) resulted in increased atherosclerosis compared with male Neu1^hypoApoe^−/− mice transplanted with male Neu1^hypoApoe^−/− BM (n = 7). Individual data are presented with mean ± S.D. * denotes p < 0.05. Scale bar = 200 μm. C and D, blood cell lipid levels in Neu1^hypoApoe^−/− mice transplanted with Apoe^−/− or Neu1^hypoApoe^−/− BM. Blood cell cholesterol (C) and TG (D) levels in BM-transplanted mice in which male Neu1^hypoApoe^−/− mice were transplanted with Neu1^hypoApoe^−/− (n = 6) or Apoe^−/− (n = 8) BM. Blood cell cholesterol and TG levels were not significantly different between Neu1^hypoApoe^−/− mice transplanted with Neu1^hypoApoe^−/− BM and those transplanted with Apoe^−/− BM. The peripheral blood cell cholesterol and TG were determined using an enzymatic assay after extracting the blood cell lipids. Individual data are presented with mean ± S.D.