Figure 5.

Peripheral blood immunophenotypes in male Apoe^−/− and Neu1^hypoApoe^−/− mice. Samples were treated with ACK lysis buffer, blocked with CD16/CD32 antibody, stained with directly conjugated antibodies for the cell-surface markers, fixed, and run on an LSR II flow cytometer. A, proportions of CD3ϵ⁺ T cells, CD19⁺ B cells, and NK1.1⁺ NK cells in peripheral blood. The male Neu1^hypoApoe^−/− mice displayed increased CD3ϵ⁺ T cells but slightly reduced NK1.1⁺ NK cells. B, proportions of CD4⁺ T helper lymphocytes and CD8⁺ cytotoxic T lymphocytes subsets in the CD3ϵ⁺ gate. The male Neu1^hypoApoe^−/− mice had a significantly greater prevalence of CD4⁺ T helper lymphocytes. C, prevalence of CD115^hiLy6C^neg-lo patrolling monocytes, CD115^hiLy6C^hi inflammatory monocytes, and SSC^hiCD11b⁺CD115^loLy6C^int neutrophils. The male Neu1^hypoApoe^−/− mice exhibited subtle increases in the monocyte populations and a striking decrease in the neutrophil prevalence. Individual data are presented with mean frequency ± S.D. presented in each graph (n = 3–6 for each group).