Table 1.

Lipid analysis in serum and liver of male Apoe^−/− and Neu1^hypoApoe^−/− mice

Enzymatic assays were used to measure different lipids in the liver and serum of 7-month-old chow-fed male mice. Folch extraction was used to extract lipids from the liver, while the serum was used directly from the unfasted animals. The hepatic total and free cholesterol levels were decreased in Neu1^hypoApoe^−/− (n = 8) mice, while the hepatic cholesteryl ester concentration was increased (not significantly) compared with Apoe^−/− mice (n = 3). There was a significant decrease in serum total cholesterol, free cholesterol, and cholesteryl esters in the Neu1^hypoApoe^−/− mice (n = 8) compared with the Apoe^−/− mice (n = 3).

	Liver				Serum
	Total cholesterol	Free cholesterol	Cholesteryl esters	TG	Total cholesterol	Free cholesterol	Cholesteryl esters	TG
	mg/g liver				mm
Apoe−/−	3.62 ± 0.29	3.16 ± 0.30	0.46 ± 0.13	16.98 ± 1.52	32.24 ± 2.12	14.43 ± 2.38	17.81 ± 0.69	3.32 ± 0.19
Neu1hypoApoe−/−	2.22 ± 0.11a	1.52 ± 0.18b	0.70 ± 0.18	14.69 ± 1.74	14.97 ± 1.42a	7.45 ± 0.75a	7.52 ± 1.14a	4.38 ± 0.49

^a Values represent the mean of biological replicates ± S.E., p < 0.001.

^b Values represent the mean of biological replicates ± S.E., p < 0.05.