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. 2018 Aug 8;293(38):14572–14584. doi: 10.1074/jbc.RA118.003373

Figure 1.

Figure 1.

Expression of NKX2-3 and p21 during tooth morphogenesis. A, in samples obtained on E14, differentially expressed genes were identified by microarray analysis of teeth, and the results were compared with whole embryos. Highlighted plot, Nkx2-3. Red and blue plots, up- and down-regulated genes, respectively. B, qRT-PCR analysis of Nkx2-3 expression in tooth, heart, submandibular gland, skin, lung, eye, kidney, hair, brain, and stomach samples from E14 embryos after normalization to Gapdh mRNA expression. C, qRT-PCR analysis of Nkx2-3 expression in teeth obtained from E11 to P7 after normalization to Gapdh mRNA expression. D, qRT-PCR analysis of Nkx2-3, Krt14, and Vim expressions in tooth epithelium and mesenchyme. E, NKX2-3 (green) expression in E13, E14, and E16 mice, detected by immunocytochemistry. p21 (green) expression in E13, E14, and E16 mice, detected by immunocytochemistry. Perlecan (red) was used to detect the basement membrane. Nuclei were stained using DAPI (blue). Error bars, S.D. Scale bars, 50 μm.