Figure 3.
Influence of WTA sugar modifications on GlpQ activity. A, 2.5 mm WTA was incubated with 5 μg of recombinant MBP-GlpQ in the presence of 5 mm CaCl2. Means and standard deviations from at least three independent experiments done in triplicates are shown. Significant differences between PS187 WT and PS187 GN1 WTA were calculated with Student's t test. ****, p < 0.0001. B, extracted ion chromatograms of WTA from S. aureus PS187 and nonglycosylated mutant GN1 before (Buffer, light gray) and after treatment with MBP-GlpQ, analyzed by LC/MS. The diagrams show calculated (c.m.) and observed masses (o.m.) of GroP. Data were plotted relative to the intensity of the GroP peak of S. aureus GN1 to allow comparison.