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Activity of GlpQ visualized by WTA-PAGE. A and B, 1 mm WTA from parental S. aureus PS187 WT (PS, A) or nonglycosylated GN1 mutant strain (B) was incubated for 1 h or 16 h at room temperature with purified GlpQ or with MBP as a negative control in the presence of 5 mm CaCl2.
