Figure 1.

Mitochondria-targeted drugs inhibit colon cancer cell proliferation. A–D, HCT116 (A and C) and HT-29 (B and D) colon cancer cell lines were treated with increasing concentrations of Mito-CP (0–10 μm) or Mito-Met₁₀ (0–100 μm). Images were acquired in real time every 2 h over a 6-day period using the IncuCyte S3 and representative phase contrast images are shown. Percent confluency (% confluency) was used as a readout for proliferation. A graphical representation of the dose response on cell confluence is expressed as a change in percentage of cell confluence. Values are mean ± S.E., n = 3; a two-way repeated measures ANOVA demonstrated p ≤ 0.0001.