Figure 4.

MTD treatment shifts the phosphorylation status of ULK1 to promote mitophagy. A, HCT116 cells were treated with DMSO (V, vehicle) or increasing concentrations of Mito-Met₁₀ or Mito-CP for 24 h. Protein lysates (10 μg/lane) were resolved by SDS-PAGE and probed with antiserum directed toward phospho-Ser-757–ULK1, total ULK1, or β-tubulin (A) or phospho-Ser-317–ULK1, total ULK1, or β-tubulin (B). Immunoblots were quantitated and represented graphically below each set of blots. ** denotes p ≤ 0.01. Values are mean ± S.E. n = 3. Immunoblot analyses of AKT analyzed concurrently with ULK are shown in supporting Fig. S4.