Figure 7.
Pharmacological inhibition of the AMPK signaling pathway provides partial resistance to MTD treatment. A, HCT116 cells were treated (+) with 0.3 μm compound C (Cpd C) or remained untreated (−), for 48 h prior to incubation with DMSO (Vehicle), 50 μm Mito-Met10, or 1 μm Mito-CP. Cell images were acquired in real time on the IncuCyte S3 and percent confluency (% confluency) was used as a readout for proliferation after 3 days of MTD treatment. B, representative images of control and MTD-treated cells are shown. * denotes p ≤ 0.05, ** denotes p ≤ 0.01. Values are mean ± S.E. n = 3.