Table 5.
Quality of fit values
| Data set | Filament assembly model | Number of reactions fit (see Table 2) | Number of data points simulated and fit to experimental data | Number of parameters fita | χ2/DoFb | σ with respect to the fitb |
|---|---|---|---|---|---|---|
| 1 (uncleaved DNA only) | 5EO | 5 | 425 | 13 | 1.9 | 0.011 |
| 4EO | 1.7 | 0.011 | ||||
| 4BM | 1.4 | 0.010 | ||||
| 2 (uncleaved and precleaved DNA) | 5EO | 6 | 1550 | 16 | 3.4 | 0.012 |
| 4EO | 5.2 | 0.016 | ||||
| 4BM | 2.4 | 0.010 | ||||
| 3 (uncleaved and precleaved DNA with 10 mm Ca2+) | 5EO | 4 | 1111 | 12 | 3.0 | 0.011 |
| 4EO | 3.9 | 0.013 | ||||
| 4BM | 3.5 | 0.013 |
a Rate constants, baseline, and scaling factors. Reverse rate constants for the self-annealing of PC DNA and for DNA binding by SgrAI were constrained by the calculated or measured equilibrium dissociation constant, respectively. Hence, only the forward rate constants were fit in these reactions. Both forward and reverse rate constants were fit for assembly and disassembly of SgrAI–DNA oligomers.