Figure 1.

Ligand‐induced cAMP activity in HEK‐RXFP1 cells. HEK293T cells stably expressing RXFP1 were stimulated with vehicle, forskolin, or an RXFP1 agonist for 45 minutes. (A) Time‐course of forskolin‐induced cAMP activity. (B) Concentration‐response curve for forskolin at 45 minutes. Time‐course for (C) H2 relaxin‐ and (D) ML290‐induced cAMP activity. (E) Concentration‐response curves for H2 relaxin and ML290 at 5 and 45 minutes. Time of ligand or vehicle addition is represented by an arrow. Data represent mean ± SEM of three independent experiments