Figure 4.

Blocking and reversing the CAMYEL signal in HEK‐RXFP3 cells. (A) Pre‐treatment of HEK‐RXFP3 cells with the Gα_i/o inhibitor pertussis toxin (100 ng mL⁻¹; 20 hours) abolished R3/I5‐induced inhibition of cAMP activity. (B) Treatment with the RXFP3 antagonist R3 B1‐22R after stimulation with R3/I5 (30 minutes) and forskolin showed reversal of the R3/I5 response in real time. Time of ligand or vehicle addition is represented by an arrow. Data represent the mean ± SEM of three independent experiments