Table 3.
Efficacy and potency of agonists acting at RXFP3 in CHO‐K1 cells
| Time point | Analogue 2 | Peptide 5 | |
|---|---|---|---|
| E max (% forskolin) (bottom) | 10 minutes | 22.06 ± 2.54 | 20.42 ± 2.79 |
| 45 minutes | 21.58 ± 3.93 | 29.4 ± 2.68 | |
| pEC50 | 10 minutes | 7.98 ± 0.07 | 8.39 ± 1.00 |
| 45 minutes | 8.21 ± 0.12 | 8.70 ± 0.11* |
CHO‐RXFP3 cells were stimulated with agonist for 45 minutes, with addition of forskolin four minutes after agonist addition. The efficacy (E max) and potency (pEC50) were determined at 10 and 45 minutes. Efficacy is expressed as a percentage of the response to forskolin (10 μmol L−1). Data represent mean ± SEM of three independent experiments. *P < 0.05 compared to analogue 2 (unpaired t‐test).