Table 4.

Efficacy and potency of agonists acting at RXFP3 in HEK293T cells

	Time point	Analogue 2	Peptide 5	R3/I5
E max (% forskolin) (bottom)	10 minutes	34.27 ± 9.834	39.18 ± 7.80	64.77 ± 6.23
	45 minutes	39.72 ± 10.82	50.57 ± 9.44	47.97 ± 4.61
pEC50	10 minutes	8.24 ± 0.36	8.52 ± 0.32	8.30 ± 0.43
	45 minutes	Ambiguous	9.68 ± 0.65	9.32 ± 0.26

HEK‐RXFP3 cells were stimulated with agonist for 45 minutes, with addition of forskolin 5 minutes after agonist addition. The efficacy (E _max) and potency (pEC₅₀) were determined at 10 and 45 minutes. Efficacy is expressed as a percentage of the response to forskolin (10 μmol L⁻¹). Data represent mean ± SEM of three independent experiments.