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. 2018 Sep 18;9:1370. doi: 10.3389/fpls.2018.01370

FIGURE 5.

FIGURE 5

Light-hypersensitivity promotes auxin sensitivity and signaling without altering endogenous IAA levels. Treatment details as described in Figure 1. (A) Endogenous indole-3-acetic acid (IAA) levels in wild-type (WT) and high pigment-2 (hp2) fruits. (B) In vitro GUS activity assayed in WT and hp2 fruits carrying the auxin-responsive promoter DR5 fused to the GUS reporter protein (DR5::GUS and hp2-DR5::GUS). (C) Heatmap representation of the differences in mRNA levels of auxin signaling genes between the WT and hp2 fruits ripened under light or dark conditions. (D) Heatmap representing differences in mRNA levels of auxin signaling genes between light and dark samples of hp2 fruits at each sampling time. The relative transcript values are presented in Supplementary Figures 7, 8. (E) Relative mRNA levels of auxin-responsive genes in WT and hp2 fruits treated with 100 μM IAA for 6 h. Data are means (±SE) of at least three biological replicates. Different letters indicate statistically significant differences (Tukey’s test, p < 0.05) within each genotype (in A,B) or among all data (in E). In (A,B), asterisks indicate statistically significant differences (Student’s t-test, p < 0.05) between genotypes. MG, mature green; Bk, Breaker; Aux/IAA, auxin/indole-3-acetic acid; ARF, auxin response factor.