Table 3.

GnRH neuron excitability parameters and AP kinetics for each treatment and cycle stage in females

Parameters	Diestrus			Estrus			Overall ANOVA (F value)
	Saline	KA-long	KA-reg.	Saline	KA-long	KA-reg.	Treatment	Cycle Stage
AP threshold (mV)	-28 + 3.6	-39.1 + 2.5*	-40.8 + 1.7**	-35.8 + 2.8#	-44.0 + 2.0*	-40.4 + 2.1	6.57*	4.54*
Input resistance (MΩ)	791.5 + 73.0	852.3 + 62.3	812.8 + 75.3	766.8 + 85.9	965.0 + 97.1	796.0 + 73.1	1.37	0.43
Capacitance (pF)	18.5 + 2.7	14.8 + 0.7	16.5 + 1.6	17.9 + 2.0	16.1 + 0.8	18.5 + 1.1	0.63	1.21
τ (ms)	34.1 + 2.4	40.4 + 3.4	44.7 + 5.2	45.9 + 2.9#	65.3 + 6.6*#	51.7 + 3.9	4.45*	17.02**
Latency to firing (ms)	0.57 + 0.07	0.47 + 0.05	0.48 + 0.08	0.50 + 0.07	0.36 + 0.08*	0.43 + 0.06	1.66	2.40
ISI first 10 spikes (ms)	10.0 + 0.3	9.1 + 0.2	9.6 + 0.4	9.1 + 0.4	9.8 + 0.5	9.2 + 0.3	2.71	0.50
Ins. freq. first 10 spikes (Hz)	112.9 + 6.1	122.3 + 4.1	117.9 + 6.0	130.3 + 7.2	113.8 + 5.7	119.8 + 5.1	0.10	0.50
FWHM (ms)	2.1 + 0.1	2.2 + 0.1	2.3 + 0.2	2.4 + 0.1	2.5 + 0.2	2.4 + 0.1	0.99	0.43
AHP (pA)	35.0 + 3.2	29.7 + 2.0	27.9 + 1.8	30.2 + 1.4	28.7 + 2.0	27.9 + 2.0	2.52	0.33
Time to AHP (ms)	3.8 + 0.1	3.8 + 0.1	3.7 + 0.2	4.1 + 0.2	3.9 + 0.3	3.8 + 0.3	0.19	0.76
Max rise slope	171.3 + 11.7	182.7 + 12.6	155.8 + 14.4	155.5 + 12.2	157.1 + 13.0	163.1 + 15.4	0.58	1.66
Max decay slope	-80.9 + 3.7	-78.8 + 4.2	-67.4 + 6.5	-74.8 + 3.8	-71.9 + 3.4	-73.2 + 3.8	1.94	0.88

ISI first 10 spikes, average ISI of the first 10 evoked spikes; Ins. freq. first 10 spikes, average instantaneous frequency of the first 10 evoked spikes; FWHM, full-width at half-maximum; AHP, afterhyperpolarization; time to AHP, time between the AP initiation and the peak of AHP; *p < 0.05, **p < 0.01 two-way ANOVA with Fisher’s LSD post hoc tests.