Skip to main content
. 2018 Sep 18;9:2229. doi: 10.3389/fmicb.2018.02229

FIGURE 2.

FIGURE 2

Phenotypic characterization of AaMFS54 strains. (A) Production of conidia by the wild-type (WT) and two AaMFS54 impaired mutants (Δmfs54-D18 and Δmfs54-D47) of A. alternata grown on PDA. Means followed by the same letters were not significantly different according to student t-test (P ≤ 0.05). (B) Growth of WT, D18 and fungal transformants from D18 protoplasts after being co-transformed with a copy of AaMFS54 and pBarks1 plasmid on PDA amended with 0.5 μg/ml fludioxonil 7 days. Several transformants (indicated by arrows) displayed WT growth, indicative of expression of AaMFS54. (C) Image of DNA fragments amplified from genomic DNA of WT, three Δmfs54 mutants (D18, D46, and D47) and four complementation strains (CP2, CP4, CP5, and CP8) with primers SSH54P and SSH54T. (D) Reverse transcriptase-PCR products from amplified from RNA samples isolated from A. alternata strains with SSH54QF1 and SSH54QR2 primers (top panel) and actin gene primers (bottom panel), indicating the expression of AaMFS54 in WT, CP5, and CP8. No product was amplified from RNA samples prepared from D18 and D47.