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. 2018 Sep 18;9:2229. doi: 10.3389/fmicb.2018.02229

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Copyright © 2018 Lin, Yu, Chen, Tsai and Chung.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The Alternaria alternata major facilitator superfamily transporter (AaMFS54) is required for resistance to xenobiotics and fungicides. Image of the wild-type (WT), two Δmfs54 deletion mutants (D18 and D47), and the CP8 complementation strain grown on potato dextrose agar (PDA) amended with indicated chemicals (left panel). Only representatives are shown. Quantitative analysis of chemical sensitivity is shown on the right panel. Growth reduction was calculated as a cumulative percentage of growth of wild-type and Δmfs54 strains grown on the same plate. The data presented are the mean and standard deviation of two independent experiments with four replicates. Abbreviations: CHP, 2-chloro-5-hydroxypyridine; TIBA, 2,3,5-triiodobenzoic acid. Means followed by the same letters were not significantly different according to student t-test (P ≤ 0.05).