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. 2018 Sep 18;9:2229. doi: 10.3389/fmicb.2018.02229

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Copyright © 2018 Lin, Yu, Chen, Tsai and Chung.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The A. alternata major facilitator superfamily transporter (AaMFS54) is a virulence determinant. (A) Conidial suspensions (10⁴ conidia per ml) prepared from WT and Δmfs54 strains were inoculated by dropping 5 μl on detached calamondin leaves (n = 8). The diameter of lesions was measured 2 days post inoculation (dpi). Means followed by the same letters were not significantly different according to student t-test (P ≤ 0.05). (B) Formation of necrotic lesions (2 dpi) and fungal colonies and fluffy hyphae on necrotic lesions (4 dpi). (C) Development of necrotic lesions on detached calamondin leaves inoculated by spraying with conidial suspensions 2 dpi. The mock controls were treated with water only. The leaves were maintained in a moisture chamber for lesion development. Only representatives are shown.