FIGURE 5.

Expression of the AaMFS54 gene by Northern blot analysis. (A) RNA was purified from wild-type (WT) grown on PDA with or without chemicals. Abbreviations: TIBA, 2,3,5-triiodobenzoic acid; CHP, 2-chloro-5-hydroxypyridine; Vin, vinclozolin; and Azole, clotrimazole. (B) Detection of the AaMFS54 transcript in WT, two Δyap1 strains (YD1 and YD2) impaired at the Yap1 transcription regulator, the YCp strain re-acquiring a functional Yap1 gene, and two Δhog1 strains (HD1 and HD2) impaired at the Hog1 MAP kinase. RNA was electrophoresed on a formaldehyde-containing gel, blotted to membrane, and probed with an AaMFS54-specific probe (Figure 1B). Ribosomal RNA after being stained with ethidium bromide is used to indicate the loading of the samples.