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. 2018 Jul 18;27(8):1476–1490. doi: 10.1002/pro.3441

Figure 2.

Figure 2

HPLC profiles of the expressed and cleaved fusion proteins carrying 14 of the 19 zebrafish GEM peptides (Fig. 1). Each fusion protein contains a thioredoxin tag and a His‐tag, which are separated from the GEM peptide after proteolytic cleavage (see the Experimental Section). The numbered peaks indicate the collected HPLC fractions and the red‐colored peak was chosen for further NMR analyses (Fig. 3) after a preliminary screening of collected fractions. (A) HPLC elution profiles for the purification of seven progranulin Aa GEMs: AaA, AaC, AaD, AaE, AaF, AaG, and AaH. Fractions for NMR spectra screening included 3 and 5 of AaA, 3 of AaC, 1 and 2 of AaD, 1, 3, and 4 of AaE, 2, 4, 5, and 7 of AaF, 1 of AaG, and 1 and 3 of AaH. (B) Elution profiles of five GEMs from progranulin Ab, AbA, AbB, AbE, AbF, and AbG and the two GEMs, Grn1 and Grn2, from progranulins 1 and 2, respectively. Fractions for NMR screening included 1 of AbA, 3, 4, and 5 of AaB, 2, 3, and 4 of AbE, 2 and 4 of AbF, 1 of AbG, 1 of Grn1 and Grn2.