Table 2. . Summary on the analytical performance of published plasma paracetamol LC–MS/MS quantitation methods.

Year of study	Method description	Sample volume (μl)	LLOQ (mg/l)	LC run-time (min)	Linearity (mg/l)	%CV (exclude LLOQ)		Ref.
						Intra-assay	Interassay
2017	Protein precipitation	20	0.125	5.5	0.125–50	<1.0	<1.4	Current study
2015	Protein precipitation, vacuum drying and reconstitution	10	0.05	20	0.05–50	<7.1%	<7.2%	[26]
2013	Protein precipitation	50	0.003	4.2	0.003–20	<4.9	<8.7	[23]
2013	Protein precipitation, vacuum drying and reconstitution	25	0.25	10	0.25–20	<2.9	<6.3%	[25]
2012	Protein precipitation, vacuum drying and reconstitution	50	0.020	18	0.02–10	<3.9%†		[24]
2011	Protein precipitation	100	0.03	NA	0.03–9	<8.4%†		[43]
2010	Protein precipitation	50	0.01	6	0.01–5	<6.8	<10.0	[28]
2008	Protein precipitation	50	0.012	6	0.012–25	<10.8	<13.0	[44]
2006	Protein precipitation, followed by SPE	100	5	70	5–100	<5.7	<6.3	[45]
2003	Protein precipitation	NA	0.01	25	0.01–5	<3.4%†		[46]

^†Not specified.

LLOQ: Lower limit of quantitation; NA: Not available.