Fig. 1.

Protein sequence alignment and phylogenetic tree of CsLsi2 along with other identified silicon transporters. (A) Protein sequence alignment of Lsi2s from cucumber (Cucumis sativus) (Cs), pumpkin (Cucurbita moschata) (Cm), barley (Hordeum vulgare) (Hv), rice (Oryza sativa) (Os), maize (Zea mays) (Zm) and horsetail (Equisetum arvense) (Ea). (B) Phylogenetic tree of Lsi2s from different plant species and rat. Protein sequence alignment was performed with DNAMAN v.6; the phylogenetic tree was constructed using the neighbor-joining algorithm with MEGA v.6. The scale bar represents a substitution distance of 0.2. Gene IDs for each of the silicon transporter proteins are listed in Supplementary Data Table S1.