Fig. 4.

Transport activity of CsLsi2 in Xenopus laevis oocytes. (A) Silicon efflux transport activity of CsLsi2. CsLsi2 cRNA and 1.0 mm Ge or just 1.0 mm Ge was injected into Xenopus oocytes. The oocytes were cultured in MBS, and the released Ge was measured to determine the transport activity. Cold (4 °C) treatment was applied to determine whether the efflux transport was energy-dependent. (B) Silicon influx transport activity of CsLsi2. Xenopus oocytes injected with CsLsi2 cRNA or water were cultured in MBS containing 1 mm Ge for 30 min, after which the absorbed Ge in Xenopus oocytes was measured. Values are means ± s.d. (n = 3). Different lower-case letters indicate a significant difference at P < 0.05.