Fig. 3.

Assessment of autophagy features of the 14G2a-treated IMR-32 cells by TEM. IMR-32 cells were pre-treated with 10 μM CQ for 1.5 h and subsequently treated with the 14G2a mAb or PBS for 48 h, and visualized by TEM. The representative images account for exemplary photos of cells selected from at least 20 other photos of cells visualized under the microscope for each type of treatment. Autophagosomes (arrows) and residual bodies (arrowheads) are scattered along a cytoplasm. Scale bar 2 μm. N—nucleus, M—mitochondria. C—control, PBS-treated cells; 14G2a—the 14G2a mAb-treated cells; CQ—chloroquine-treated cells