Fig. 6.

Effects of stable silencing of the PHLDA1 gene on expression of autophagy and apoptosis-associated proteins in the 14G2a mAb-treated CHP-134 clones. Relative expression of LC3A/B-II (a), LC3A/B-I (b), ATG7 (c), ATG3 (d), P62 (e), Beclin-1 (f), cleaved caspase 3 (g), and cleaved PARP (h) proteins was assessed in selected PHLDA1-silenced (S6, S17), Mock3, and WT cells. Mean values for the 14G2a mAb-treated CHP-134 are presented as grey bars and calculated versus control values (white bars) for WT cells (black baseline). i Representative immunoblots are presented. j Relative activities of caspase 3 and 7 were calculated as caspase 3 and 7 activities at day 3 after treatment and divided by caspase 3 and 7 activities at day 1 in PHLDA1-silenced (S6, S17), Mock3 cells, and calculated versus control values for WT cells (black baseline). P-values for t test were as follow: *p < 0.05