Table 1.

List of MAVE studies, their respective target spaces, and achieved levels of coverage

Reference	Target	Search space	Coverage (%)
Fowler et al. (2010)	YAP65	WW domain	$\sim 100$
Ernst et al. (2010)	Synthetic PDZ domain	10 AAs	$\sim 100$
Hietpas et al. (2011)	Hsp90	9 AAs	$\sim 100$
Fujino et al. (2012)	Fab antibody fragment	Fragment	79
Adkar et al. (2012)	Ccdb	whole protein	$<74$
McLaughlin et al. (2012)	PSD95	PDZ domain	$\sim 100$
Schlinkmann et al. (2012)	GPCR	Whole protein	$\sim 90$
Whitehead et al. (2012)	Synthetic protein	51AA (whole protein)	99
Traxlmayr et al. (2012)	IgG1	CH2/CH3 domains	$<50$
Araya et al. (2012)	YAP65	WW domain	$\sim 100$
Deng et al. (2012)	TEM1	Whole protein	$\sim 80$
Kwasnieski et al. (2012)	Rhodopsin promoter	52 bp cis-regulatory element	$\sim 100$
Wu et al. (2013)	Neuraminidase	SNP accessible	$<50$
Roscoe et al. (2013)	Ubiquitin	Whole protein	$\sim 95$
Starita et al. (2013)	Ub.E3 E4B	Whole protein	$\sim 50$
Procko et al. (2013)	Synthetic protein	60 AA	$\sim 100$
Tinberg et al. (2013)	Synthetic protein	40AA	90
Jiang et al. (2013)	Hsp90	Substrate binding loop	$\sim 100$
Kim et al. (2013)	Mat alpha	Degron region	$<50$
Melamed et al. (2013)	Pab1	RRM domain	$\sim 90$
Forsyth et al. (2013)	Antibody for EGFR	Whole protein	$\sim 99$
Jacquier et al. (2013)	TEM1	whole protein	64
Hietpas et al. (2013)	Hsp90	pos. 528–590	$\sim 100$
Liachko et al. (2013)	ARS1	100 bp	$\sim 100$
Wagenaar et al. (2014)	BRAF	77 AAs	99.65
Firnberg et al. (2014)	TEM1 $\mathit{\beta }$-lactamase	Whole protein	$\sim 95$
Olson et al. (2014)	G-protein (GB1)	IgG-binding domain	$\sim 95$
Melnikov et al. (2014)	APH(3’)II (kinase)	Whole protein	$\sim 100$
Bloom (2014)	Influenza nucleoprotein	Whole protein	$>75$
Thyagarajan and Bloom (2014)	Influenza hemagglutinin	Whole protein	$\sim 85$
Qi et al. (2014)	NS5A	IA domain	$\sim 100$
Roscoe and Bolon (2014)	Ubiquitin	Whole protein	$\sim 95$
Reich et al. (2015)	Bcl-$x_L$ ligands	Peptide library	N/A
Stiffler et al. (2015)	TEM1 $\mathit{\beta }$-Lactamase	Whole protein	$\sim 100$
Doud et al. (2015)	Influenza nucleoprotein	Whole protein	$\sim 100$
Kitzman et al. (2015)	Gal4	DB domain	$\sim 99$
Starita et al. (2015)	BRCA1	RING domain	$\sim 80$
Rockah-Shmuel et al. (2015)	M.HaeIII	Whole protein	38
Wu et al. (2015)	PA	Whole protein	94
Mishra et al. (2016)	Hsp90	ATPase domain	$\sim 99$
Doud and Bloom (2016)	Hemagglutinin	Whole protein	$<97$
Mavor et al. (2016)	Ubiquitin	Whole protein	$\sim 99$
Majithia et al. (2016)	PPAR$\mathit{\gamma }$	Whole protein	$\sim 99$
Julien et al. (2016)	FAS/CD95	Exon 6	95
Li et al. (2016)	tRNA Arg-CCU	whole gene	$\sim 100$
Sarkisyan et al. (2016)	GFP	whole protein	$\sim 100$
Tripathi et al. (2016)	CcdB	whole protein	87
Puchta et al. (2016)	snoRNA U3	pos. 7-333	$\sim 100$
Brenan et al. (2016)	Mapk1/Erk2	Whole protein	99
Steinberg and Ostermeier (2016)	TEM1	Whole protein	39–50
Hoggard et al. (2016)	miniARS317/301	$153+135$ bp	$\sim 100$
Ma et al. (2017)	BCR-ABL	8AAs	$\sim 100$
Matreyek et al. (2017)	GFP	whole protein	$\sim 60$
Klesmith et al. (2017)	TEM1,LGK	whole proteins	$\sim 70$
Chan et al. (2017)	IGPS	8 $\mathit{\beta }$-strands	$\sim 95$
Bandaru et al. (2017)	H-Ras	pos. 2–166	$\sim 100$
Weile et al. (2017)	UBE2I,SUMO1,TPK1,CALM1/2/3	whole proteins	100
Mighell et al. (2018)	PTEN	Whole protein	$\sim 95$
Plesa et al. (2018)	PPAT	Whole protein	$\sim 95$
Matreyek et al. (2018)	PTEN,TPMT	Whole protein	$\sim 60$
Ke et al. (2018)	DHFR	exon 5	$\sim 100$
Starita et al. (2018)	BRCA1	302 AAs	$<50$
Kotler et al. (2018)	TP53	DNA-binding domain	$\sim 85$