Fig. 4.

ESCRT-II mutants have normal telomeres in S. cerevisiae W303 cells. a Southern blot as described in Fig. 2c. b Telomere southern blot of WT, vps25Δ, vps36Δ and snf8Δ mutations in S288C and W303 genetic backgrounds. S288C strains are from the deletion database. Southern blot performed as described in Fig. 2c, except that the cells were cultured at 30 °C. In each panel, all lanes are from the same gel and vertical dashed lines represent the place where the gel was cut and pasted for presentation purposes