Fig. 3.

Combinatorial biosynthesis in N. benthamiana. a Production of diterpene scaffolds in N. benthamiana though combination of pairs of class II (inner circle, blue) and class I (inner circle, green) cyclases. Bonds in a specified stereochemical configuration formed through stereoselective controlled synthesis are highlighted in red. Bonds in a specified stereochemical configuration for which there is no biosynthetic access to the other configuration are shown in black. The a and b suffixes indicate (+) and ent configurations, respectively, as dictated by the class II diterpene synthases. Diterpenes with unknown structure are shaded in grey. *Clerodane diterpenes. b Production of oxygenated triterpenes. The products of individual P450s acting upon β-amyrin (centre) are shown in the inner blue circle. The major combinatorial products arising from pairs of P450s are shown in the outer circle.

a adapted from Andersen-Ranberg et al. (2016), b adapted from Reed et al. (2017)