FIG 5.

FlgJ-SltF interactions. (A) Coimmunoprecipitation of wild type SltF and the two mutant versions of SltFΔ95 and SltFΔ47 in the presence or absence of FlgJ. The proteins were used at a concentration of 0.14 μM, and anti-His polyclonal antibodies were used to detect the proteins. (B) Far-Western blot assay with 17.5% SDS-PAGE gels was carried out with the wild type (SltF) and mutants (SltFΔ95 and SltFΔ47). Proteins were loaded at a concentration of 10 nM, transferred to nitrocellulose membranes, and incubated with FlgJ at a concentration of 17 μg/ml. Anti-FlgJ antibodies were used at a 1:1,000 dilution.