FIG 8.

SagB hydrolase is not required for S. aureus ESS secretion. (A) Cultures of S. aureus USA300 (WT), essB, sagB(vector), and sagB(psagB) variants were grown and fractionated as described in the legend for Fig. 2B. Samples were subjected to immunoblot analyses using rabbit polyclonal antibodies specific for EssH, EsxC, and L6. A representative experiment is shown. (B) Quantification of EsxC in cell and medium fractions was performed by quantifying band densities from three independent experiments as described in the legend for Fig. 6C.