Table 2.
Primary antibodies used for western blot (WB) and immunohistochemistry (IHC). RALDH, retinaldehyde dehydrogenase; RAR, retinoic acid receptor; GFAP, glial fibrillary acidic protein
| Antibody | Host | WB dilution | IHC dilution | Predicted molecular weight (kDa) | Source | Catalog # |
|---|---|---|---|---|---|---|
| Anti-RALDH1 | Rabbit | 1:1000 | 1:300 | 54 | Abcam | ab24343 |
| Anti-RALDH2 | Rabbit | 1:3000 | – | 55 | Millipore | ABN420 |
| Anti-RARα | Goat | 1:1500 | – | 51 | Abcam | ab28767 |
| Anti-RARα | Rabbit | – | 1:300 | – | Santa Cruz | sc-551 |
| Anti-RARβ | Rabbit | 1:500 | – | 50 | Abcam | ab53161 |
| Anti-S-antigen | Mouse | – | 1:50 | – | Santa Cruz | sc-166383 |
| Anti-GFAP | Mouse | – | 1:300 | – | Sigma-Aldrich | G3893 |
| Anti-ERK1/2 | Rabbit | 1:2000 | – | 44/42 | Cell Signaling | 9102 |
| Anti-phospho-ERK1/2 | Rabbit | 1:4000 | – | 44/42 | Cell Signaling | 4370 |
| Anti-Akt1/2/3 | Rabbit | 1:1000 | – | 56/62 | Santa Cruz | sc-8312 |
| Anti-phospho-Akt | Rabbit | 1:1000 | – | 60 | Cell Signaling | 9271 |