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. 2018 Jul 12;30(10):457–470. doi: 10.1093/intimm/dxy045

Fig. 7.

Fig. 7.

SOCS3 neutralization. (A) SOCS3 and β-actin western blot of day 9 (D9) cultured BMDCs and D9 BMDCs treated with conjunctival conditioned media (CjCM) and RA. (B) Compared to a control scrambled sequence, SOCS3 siRNA inhibited expression of SOCS3 transcripts at 24 h in BMDCs with or without LPS stimulation measured by RT–PCR. (C) SOCS3 western blot of LPS-stimulated BMDCs after 24-h treatment with control scrambled sequence or SOCS3 siRNA LPS (left) and the SOCS3/β-actin ratio measured from the western blot (right). (D) SOCS3 gene silencing reversed the suppressive effects of CjCM and RA on IL-12 expression in BMDCs. Untreated (UT) versus LPS within groups: *P < 0.05, **P < 0.01, ***P < 0.005; between-group (D9) comparisons: ††P < 0.01, †††P < 0.005. (E) CjCM suppressed expression of IL-12 in BMDCs stimulated with LPS or hyperosmolar (450mOsm) culture media.