Anti-apoptosis effect of the combination treatment with CMP and 5-FU on liver tissues. (A) Western blot analysis of P38, p-P38, p-JNK, Bax, and Bcl-2 in liver tissues. Groups represent, І: normal mice + saline; ІІ: model mice + saline; ІІІ: model mice + 5-FU 25 mg/kg; ІV: model mice + 5-FU 25 mg/kg + CMP 50 mg/kg; V: model mice + 5-FU 25 mg/kg + CMP 100 mg/kg. (B) Semi-quantitative analysis of western blot. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01 vs. Normal group; #
p < 0.05,
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p < 0.01 vs. Model group; $
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p < 0.001 vs. 5-FU group. (C,D) Immunohistochemical staining of P38 and p-P38 in liver tissues, respectively (magnification 400×). Groups represent, a: normal mice + saline; b: model mice + saline; c: model mice + 5-FU 25 mg/kg; d: model mice + 5-FU 25 mg/kg + CMP 50 mg/kg; e: model mice + 5-FU 25 mg/kg + CMP 100 mg/kg.