FIG 6.

Ultrastructural examination of host mitochondria associated with the PV of Toxoplasma with supplemented OA. (A) EM of a PV of Toxoplasma in HFF exposed for 24 h to 0.2 mM OA, illustrating host mitochondria distributed all around the PV. Host mitochondria associated with the PV show severe cytopathies (arrows in panels a and b), in contrast to host mitochondria in the cytoplasm (double arrows in b). hm, host mitochondrion; hc, host cell; hLD, host LD; P, parasite. Bars, 500 nm. (B) EM of an abnormally swollen host mitochondrion associated with the PV (a), in accordance with IFA observations with Tom20 staining of infected HFF treated for 24 h with 0.2 mM OA (b). Bars, 250 nm. (C) EM of large host mitochondria associated with the PV upon incubation with 0.4 mM OA. The mitochondria exhibit distorted cristae or cristae with angular features (a and b). Bars, 400 nm.