TABLE 4.
Antibiotic susceptibility profiles for E. coli and K. pneumoniae spontaneous mutants selected on LYS228a
| Strain | Gene mutated (aa substitution) | MIC (μg/ml) |
||||||
|---|---|---|---|---|---|---|---|---|
| LYS228 | ATM | CAZ | IPM | LVX | CHL | TGC | ||
| E. coli | ||||||||
| NB27119 (minus TEM-12) | Parent (minus TEM-12) | 0.125 | 0.125 | 0.25 | 0.25 | 0.06 | 8 | 0.5 |
| NB27119-JBA0001* (TEM-12 minus) | baeS (12-bp deletion)** | 2 | 1 | 1 | 0.125 | 0.06 | 8 | 0.5 |
| NB27119-JBA0002 (TEM-12 minus) | baeS(V270G) | 1 | 0.5 | 0.5 | 0.125 | 0.06 | 8 | 0.5 |
| NB27119-CDJ0138 | acrD promoter region | 4 | 2 | 0.5 | 0.125 | 0.03 | 4 | 0.25 |
| NB27119 (TEM-12) | Parent (TEM-12) | 0.25 | 2 | 32 | 0.25 | 0.06 | >64 | 0.25 |
| NB27119-CDJ0129 (TEM-12) | baeS(D156Y) | 2 | 2 | 32 | 0.125 | 0.06 | >64 | 0.25 |
| NB27119-CDJ0130 (TEM-12) | baeS(I170 M) | 2 | 2 | 32 | 0.125 | 0.06 | >64 | 0.25 |
| K. pneumoniae | ||||||||
| NB29002 | Parent | 0.06 | 0.06 | 1 | 0.25 | 0.06 | 8 | 0.5 |
| NB29002-JBA0001* | ramR (36-bp deletion) | 0.5 | 0.25 | 1 | 0.25 | 1 | >64 | 8 |
| NB29002-JBA0010* | ramR (406-bp deletion)**, selected on TGC | 1 | 0.5 | 2 | 0.25 | 1 | >64 | 8 |
| NB29002-JBA0004* | baeS(V295G)** | 1 | 0.5 | 1 | 0.25 | 0.06 | 8 | 0.5 |
| NB29002-JBA0008* | baeS(V295G)** | 2 | 0.5 | 1 | 0.5 | ≤0.03 | 8 | 0.5 |
| NB29002-JBA0007* | cpxA(L57V); VK055_RS01255; (I58T) | 2 | 1 | 1 | 0.5 | 0.125 | 16 | 1 |
| NB29002-JWK0175 | cpxA(L57V) | 0.5 | 0.25 | 1 | 0.5 | 0.06 | 8 | 0.5 |
| NB29002-JWK0180 | VK055_RS01255; (I58T) | 0.125 | 0.06 | 0.25 | 0.5 | 0.06 | 8 | 0.5 |
| NB29002-CDY0308 | cpxA(L57V); VK055_RS01255; (I58T) | 0.125 | 0.06 | 0.5 | 0.125 | 0.125 | 8 | 0.5 |
| NB29002-CDY0309 | cpxA(L57V) VK055_RS01255; (I58T) baeR(E19K) | 0.5 | 0.25 | 1 | 0.5 | 0.06 | 8 | 0.5 |
a
E. coli mutants were selected on 0.5 μg/ml LYS228, and K. pneumoniae mutants were selected on 0.25 μg/ml LYS228. An asterisk indicates mutation identified by whole-genome sequencing. Two asterisks indicate this was the only mutation identified in this mutant by genome sequencing. All mutations were confirmed by PCR and sequencing.