FIG 4.

Leakiness of the blood vessels in the skin of CL-infected mice at different time points postinfection and controls. After administration of Evans blue (200 μl 0.5% i.v.), the amount of the blue dye per gram of tissue was determined in the lesion (●) and healthy control skin (○) for all animals. CL-infected mice with skin lesions were dosed with Evans blue at the time when a papule, an initial nodule, or an established nodule was present on the rump (5, 10, and 20 days after L. major infection, respectively, and 15, 30, and 45 days after L. mexicana infection, respectively). Controls for skin inflammation were uninfected mice (Uninf), pseudolesion (PL; mice with carrageenan-induced inflammatory skin initial nodule), and healed lesion (HL; mice with paromomycin-cured L. major initial nodule). Data are shown as the means ± SEM (n = 3 to 5 per group). Statistical analysis was determined with a 2-way ANOVA, followed by Šidák multiple-comparison test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. The picture shows L. major-infected mice (day 10) after 30 min after administration of Evans blue (i.v.). The arrows point at the blue coloration of the CL lesions (before skin sample collection, left photo) as well as intense blue staining of the underlying thoracolumbar fascia (after skin sample collection, right photo).