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ϕNPV1 adsorption assays. Overnight cultures were diluted 1:5 in fresh BHI and equilibrated at 37°C. ϕNPV1 was added at an MOI of 10−2. After 15 min of incubation, 1 ml of each culture was centrifuged, and the titer of the supernatant was determined with the phage spot assay. A medium with only phage was used as the control. Percent adsorption was calculated as [(PFU culture − PFU control)/PFU control] × 100. The data are the average of the results from three independent trials.
